Atcc hl-60

(f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells(CCL-240; ATCC, Manassas, VA) were maintained at 37°C in Iscove modifiedDulbecco medium supplemented with 10% (vol/vol) FBS and 1% (vol/vol) penicillin-streptomycin in a humidified atmosphere of 95% air and 5% CO2 Differentiation of HL-60 cells into PMN-like cells was induced by incubating them in 1.25% DMSO for 6 days. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research.

26.04.2021 Atcc hl-60

Pricing. Characteristics. Derivation. HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood Clone 15 HL-60 (ATCC® CRL-1964™). This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with DNA purified from ATCC CCL-240, HL-60, acute promyelocytic leukemia cell line . MoreLess. Pricing.

Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018

The following ATCC cell lines can serve as models for normal differentiation: Date Updated: 6/5/2018; References Gallagher R, et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979.

Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p

Penicillin-Streptomycin Solution (100X) (Cellgro Cat# 30-002 … ATCC HL-60, Promyelocytic Leukemia, Frozen . Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.

Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service … hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes.

Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte) Article Snippet: HL-60 culture under DMSO and ATRA exposure HL-60 cells (CCL-240, ATCC, Manassas, VA) were either cultured in RPMI 1640 containing with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin/streptomycin antibiotics or RPMI 1640 containing 2% FBS, 2% Nutridoma and 5 mL % penicillin/streptomycin antibiotics at 37 °C in 5% Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA).

… I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS (non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a density if 4*105 cells/ml. Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 Page .

HL-60. Human. Leukemia, acute myeloid, M2. Feb 23, 2018 In addition, curcumin induced G1 phase arrest in HL-60 cells and G2/M the American Type Culture Collection (ATCC; Manassas, VA, USA). The ATCC HCoV-OC43 strain (VR-759) was grown on the HRT-18 rectal tumor HL-60. Human. Bone marrow. Monocyte.

HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells(CCL-240; ATCC, Manassas, VA) were maintained at 37°C in Iscove modifiedDulbecco medium supplemented with 10% (vol/vol) FBS and 1% (vol/vol) penicillin-streptomycin in a humidified atmosphere of 95% air and 5% CO2 Differentiation of HL-60 cells into PMN-like cells was induced by incubating them in 1.25% DMSO for 6 days. Aliases: HL-60. Disease ATCC Gender Female HL60 sequenced and profiled in following datasets. Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte) HL-60 has recently been reported to contain a sequence similar to that of the human endogenous retrovirus H/F-expressing cell line Reh (ATCC CRL-8286) (70), although there is currently no evidence that human endogenous retroviruses are expressed.

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ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes.

To ATCC Valued Customers, HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at a concentration of 190 nM. hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT.